Generation of expression calls for RNA-seq data
نویسندگان
چکیده
منابع مشابه
Differential Expression Analysis for RNA-Seq Data
RNA-Seq is increasingly being used for gene expression profiling. In this approach, next-generation sequencing (NGS) platforms are used for sequencing. Due to highly parallel nature, millions of reads are generated in a short time and at low cost. Therefore analysis of the data is a major challenge and development of statistical and computational methods is essential for drawing meaningful conc...
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This study was performed to determine the digital expression profile of different genes expressed in Holstein and Cholistani breeds as well as to evaluate the performance of predicted proteins derived from differentially expressed genes between these two breeds using RNA-Seq data. For this purpose, the whole mRNA sequence for a blood sample of American Holstein and Pakistani Cholistani cattle p...
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Accurate allele specific expression estimation requires the availability of a diploid transcriptome, which makes it a challenging problem. Most existing methods rely on simple counting of alleles coverage at heterozygous Single Nucleotide Polymorphic sites. In this work, we present RNA-PhASE, a pipeline for Allele Specific gene and isoform Expression estimation from RNA-Seq Reads. The pipeline ...
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Grade of membership models, also known as “admixture models”, “topic models” or “Latent Dirichlet Allocation”, are a generalization of cluster models that allow each sample to have membership in multiple clusters. These models are widely used in population genetics to model admixed individuals who have ancestry from multiple “populations”, and in natural language processing to model documents h...
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3 RNA–Seq data preprocessing 2 3.1 Creation of a sample metadata table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 3.2 Quality control commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.3 Alignment of reads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.4 Sorting and in...
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ژورنال
عنوان ژورنال: EMBnet.journal
سال: 2013
ISSN: 2226-6089
DOI: 10.14806/ej.19.a.670